Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages
This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods. 12 figs.
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- Assignee:
- Associated Universities, Inc., Washington, DC (United States)
- Patent Number(s):
- US 5,824,528/A/
- Application Number:
- PAN: 8-292,081; CNN: Grant GM21872
- OSTI ID:
- 675831
- Resource Relation:
- Other Information: PBD: 20 Oct 1998
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages
Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages