Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages

Studier, F William; Dubendorff, John W

Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages

Patent · 31 December 1997

OSTI ID:871914

Studier, F William ^[1]; Dubendorff, John W ^[2]

Stony Brook, NY
Sound Beach, NY

This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY

Assignee:: Associated Universities, Inc. (Washington, DC)

Patent Number(s):: US 5824528

OSTI ID:: 871914

Country of Publication:: United States

Language:: English

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Related Subjects

/435/999/
achieved
activity
autogene
autogenes
autogenes encoding
bacteriophages
cell
cell growth
cloning
combining
controlled
described
encoded
encoding
encoding rna
erases
expression
expression systems
genes encoding
growth
host
host cell
independent
inhibition
lac-repression
lysozyme
materials
methods
operator-t7
permit
poly
polymerase
polymerases
producing
producing selected
products
promoter
recognized
recombinant
reducing
related
related materials
relates
rna
rna poly
rna polymerase
rna polymerases
selected
sufficiently
systems
t7
t7 rna
t7-like
t7-like bacteriophages
transcribed

Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages

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