Method for construction of normalized cDNA libraries

Patent · Thu Jan 01 00:00:00 EST 1998

OSTI ID:871950

Soares, Marcelo B ^[1]; Efstratiadis, Argiris ^[2]

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1. New York, NY
2. Englewood, NJ

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.

• Research Organization: Columbia Univ., New York, NY (United States)
• DOE Contract Number: FG02-91ER61233
• Assignee: Trustees of Columbia University in City of New York (New York, NY)
• Patent Number(s): US 5830662
• OSTI ID: 871950
• Country of Publication: United States
• Language: English

Related Subjects

method
construction
normalized
cdna
libraries
provides
normalize
directional
library
constructed
vector
allows
propagation
single-stranded
circle
form
comprising
propagating
circles
generating
fragments
complementary
noncoding
sequence
produce
partial
duplexes
purifying
melting
reassociating
purified
appropriate
cot
unassociated
generated
above-described
cdna libraries
produce partial
directional cdna
single-stranded circles
cdna library
partial duplexes
normalized cdna
purified partial
single-stranded circle
unassociated single-stranded
appropriate cot
noncoding sequence
circle form
allows propagation
coding sequence
library constructed
form comprising
generating fragments
fragments complementary
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