Method for construction of normalized cDNA libraries

Patent · Tue Nov 03 00:00:00 EST 1998

OSTI ID:675824

Soares, M B; Efstratiadis, A

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3` noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries. 19 figs.

• Research Organization: Columbia Univ., New York, NY (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-91ER61233
• Assignee: Columbia Univ., New York, NY (United States)
• Patent Number(s): US 5,830,662/A/
• Application Number: PAN: 8-619,542
• OSTI ID: 675824
• Resource Relation: Other Information: PBD: 3 Nov 1998
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA-CLONING
DESIGN
DNA
DNA REPLICATION