Method for construction of normalized cDNA libraries

Patent · Sun Dec 31 23:00:00 EST 1995

OSTI ID:870244

Soares, Marcelo B ^[1]; Efstratiadis, Argiris ^[2]

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1. New York, NY
2. Englewood, NJ

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

• Research Organization: Columbia University
• DOE Contract Number: FG02-91ER61233
• Assignee: Trustees of Columbia University in City of New York (New York, NY)
• Patent Number(s): US 5482845
• OSTI ID: 870244
• Country of Publication: United States
• Language: English

Related Subjects

/435/999/
allows
allows propagation
cdna
cdna libraries
cdna library
circle
circle form
circles
coding sequence
complementary
comprising
constructed
construction
cot
directional
directional cdna
duplexes
form
form comprising
fragments
fragments complementary
generating
generating fragments
libraries
library
library constructed
melting
method
moderate
noncoding
noncoding sequence
normalize
normalized
normalized cdna
partial
partial duplexes
produce
produce partial
propagating
propagation
provides
purified
purified partial
purifying
reassociating
sequence
single-stranded
single-stranded circle
single-stranded circles
unassociated
unassociated single-stranded
vector