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Title: Method for construction of normalized cDNA libraries

Abstract

This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Inventors:
 [1];  [2]
  1. New York, NY
  2. Englewood, NJ
Publication Date:
Research Org.:
Columbia Univ., New York, NY (United States)
OSTI Identifier:
870244
Patent Number(s):
US 5482845
Assignee:
Trustees of Columbia University in City of New York (New York, NY)
DOE Contract Number:  
FG02-91ER61233
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
method; construction; normalized; cdna; libraries; provides; normalize; directional; library; constructed; vector; allows; propagation; single-stranded; circle; form; comprising; propagating; circles; generating; fragments; complementary; noncoding; sequence; produce; partial; duplexes; purifying; melting; reassociating; purified; moderate; cot; unassociated; cdna libraries; produce partial; directional cdna; single-stranded circles; cdna library; partial duplexes; normalized cdna; purified partial; single-stranded circle; unassociated single-stranded; noncoding sequence; circle form; allows propagation; coding sequence; library constructed; form comprising; generating fragments; fragments complementary; /435/999/

Citation Formats

Soares, Marcelo B, and Efstratiadis, Argiris. Method for construction of normalized cDNA libraries. United States: N. p., 1996. Web.
Soares, Marcelo B, & Efstratiadis, Argiris. Method for construction of normalized cDNA libraries. United States.
Soares, Marcelo B, and Efstratiadis, Argiris. Mon . "Method for construction of normalized cDNA libraries". United States. https://www.osti.gov/servlets/purl/870244.
@article{osti_870244,
title = {Method for construction of normalized cDNA libraries},
author = {Soares, Marcelo B and Efstratiadis, Argiris},
abstractNote = {This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

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