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Title: Recombinant cells that highly express chromosomally-integrated heterologous genes

Patent ·
OSTI ID:871892
 [1];  [1];  [1]
  1. Gainesville, FL
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Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.

Research Organization:
Univ. of Florida, Gainesville, FL (United States)
DOE Contract Number:
FG05-86ER13574
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Patent Number(s):
US 5821093
OSTI ID:
871892
Country of Publication:
United States
Language:
English

References (13)

Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12. journal November 1988
Purification and characterization of phosphatidylethanolamine N-methyltransferase from Zymomonas mobilis. journal January 1987
Isolation and characterization of mutations creating high-efficiency transcription initiation signals within the trp operon of Escherichia coli. journal January 1976
Characterization of the total extractable lipids of Zymomonas mobilis var. mobilis journal October 1982
Ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum. journal January 1992
Isolation of free ceramide from Zymomonas mobilis. journal January 1990
Lipid composition of Zymomonas mobilis: effects of ethanol and glucose. journal January 1983
Microbial tolerance to alcohols: role of the cell membrane journal February 1986
Thermal Tolerance of Zymomonas mobilis: Temperature-Induced Changes in Membrane Composition † journal January 1986
Bacterial sterol surrogates. Biosynthesis of the side-chain of bacteriohopanetetrol and of a carbocyclic pseudopentose from 13C-labelled glucose in Zymomonas mobilis journal January 1989
Transcriptional patterns for the thrS-infC-rplT operon of Escherichia coli journal March 1988
Influence of ethanol on the hopanoid content and the fatty acid pattern in batch and continuous cultures of Zymomonas mobilis journal January 1985
New method for generating deletions and gene replacements in Escherichia coli. journal January 1989

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Related Subjects

recombinant
cells
highly
express
chromosomally-integrated
heterologous
genes
host
obtained
comprise
polypeptide-encoding
polynucleotide
segment
stably
integrated
chromosome
transcriptional
control
endogenous
promoter
mutation
effects
increased
expression
resulting
enhanced
production
polypeptide
encoded
relative
absence
achieved
retained
conditions
select
displaying
comprises
example
ethanol-production
efficient
ethanol
producer
zymomonas
mobilis
escherichia
coli
enteric
bacterial
capable
converting
wide
range
biomass-derived
sugars
efficiently
zymomonas mobilis
wide range
host cells
recombinant host
host cell
recombinant cells
segment comprises
escherichia coli
heterologous genes
stably integrated
chromosomally-integrated heterologous
express chromosomally-integrated
highly express
polypeptide-encoding polynucleotide
polynucleotide segment
/435/999/