Recombinant cells that highly express chromosomally-integrated heterologous gene

Ingram, Lonnie O.; Ohta, Kazuyoshi; Wood, Brent E.

Title: Recombinant cells that highly express chromosomally-integrated heterologous gene

Patent · Tue Mar 20 00:00:00 EDT 2007

OSTI ID:1176154

Ingram, Lonnie O.; Ohta, Kazuyoshi; Wood, Brent E.

Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE; US Dept. of Agriculture (USDA)

DOE Contract Number:: FG05-86ER13574; 88-37233-3987

Assignee:: The University of Florida Research Foundations, Inc. (Gainesville, FL)

Patent Number(s):: 7,192,772

Application Number:: 09/620,060

OSTI ID:: 1176154

Resource Relation:: Patent File Date: 2000 Jul 21

Country of Publication:: United States

Language:: English

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