Recombinant cells that highly express chromosomally-integrated heterologous genes
Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol. 13 figs.
- Sponsoring Organization:
- USDOE, Washington, DC (United States); Department of Agriculture, Washington, DC (United States)
- Assignee:
- Univ. of Florida Research Foundation, Inc., Gainesville, FL (United States)
- Patent Number(s):
- US 5,821,093/A/
- Application Number:
- PAN: 8-363,868; CNN: Grant 88-37233-3987
- OSTI ID:
- 675832
- Resource Relation:
- Other Information: PBD: 13 Oct 1998
- Country of Publication:
- United States
- Language:
- English
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Recombinant cells that highly express chromosomally-integrated heterologous genes
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