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DNA complexes with dyes designed for energy transfer as fluorescent markers

Patent ·
OSTI ID:871628
 [1];  [2]
  1. Orinde, CA
  2. Albany, CA
+ Show Author Affiliations

Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.

Research Organization:
University of California
DOE Contract Number:
FG03-91ER61125
Assignee:
Regents of University of California (Berkeley, CA)
Patent Number(s):
US 5767267
OSTI ID:
871628
Country of Publication:
United States
Language:
English

References (10)

Ethidium dimer: A new reagent for the fluorimetric determination of nucleic acids journal April 1979
Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications journal January 1992
The Interaction of Intercalating Drugs with Nucleic Acids journal June 1981
Effect of B-Z transition and nucleic add structure on the conformational dynamics of bound ethidium dimer measured by hydrogen deuterium exchange kinetics journal January 1985
DNA bifunctional intercalators. 2. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer. Appendix: Numerical solution of McGhee and von Hippel equations for competing ligands journal November 1978
Design of Bifunctional Nucleic Acid Ligands book January 1990
DNA bifunctional intercalators. 1. Synthesis and conformational properties of an ethidium homodimer and of an acridine ethidium heterodimer journal November 1978
Dynamic structure of DNA complexes. Fluorometric measurement of hydrogen-deuterium exchange kinetics of DNA-bound ethidium dimer and acridine-ethidium dimer journal June 1983
High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange journal January 1991
A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels. journal May 1990

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Related Subjects

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