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DNA complexes with dyes designed for energy transfer as fluorescent markers

Patent ·
OSTI ID:869805
 [1];  [2]
  1. Orinda, CA
  2. Albany, CA
+ Show Author Affiliations
Heteromultimeric fluorophores are provided for binding to DNA, which allow for the detection of DNA in electrical separations and preparation of probes having high-fluorescent efficiencies and large Stokes shifts. In addition, by appropriate choice of fluorescent molecules, one can use a single narrow wavelength band excitation light source, while obtaining fluorescent emissions having sufficient separation to be readily discriminated.
Research Organization:
University of California
DOE Contract Number:
FG03-91ER61125
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5401847
OSTI ID:
869805
Country of Publication:
United States
Language:
English

References (8)

Ethidium dimer: A new reagent for the fluorimetric determination of nucleic acids journal April 1979
Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications journal January 1992
The Interaction of Intercalating Drugs with Nucleic Acids journal June 1981
Effect of B-Z transition and nucleic add structure on the conformational dynamics of bound ethidium dimer measured by hydrogen deuterium exchange kinetics journal January 1985
DNA bifunctional intercalators. 2. Fluorescence properties and DNA binding interaction of an ethidium homodimer and an acridine ethidium heterodimer. Appendix: Numerical solution of McGhee and von Hippel equations for competing ligands journal November 1978
DNA bifunctional intercalators. 1. Synthesis and conformational properties of an ethidium homodimer and of an acridine ethidium heterodimer journal November 1978
Dynamic structure of DNA complexes. Fluorometric measurement of hydrogen-deuterium exchange kinetics of DNA-bound ethidium dimer and acridine-ethidium dimer journal June 1983
High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange journal January 1991

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addition
allow
appropriate
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band
band excitation
binding
choice
complexes
designed
detection
discriminated
dna
dna complexes
dyes
dyes designed
efficiencies
electrical
electrical separations
emissions
energy
energy transfer
excitation
excitation light
fluorescent
fluorescent emission
fluorescent emissions
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fluorescent molecules
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heteromultimeric
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high-fluorescent
high-fluorescent efficiencies
light
light source
markers
molecules
narrow
narrow wavelength
obtaining
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preparation
probes
provided
readily
readily discriminated
separation
separations
shifts
single
single narrow
source
stokes
stokes shift
stokes shifts
sufficient
sufficient separation
transfer
wavelength
wavelength band