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Title: Single step signal group-imidazole labeling of organic phosphate groups under aqueous conditions

Patent ·
OSTI ID:870396
;

Compounds and methods for single step, covalent labeling of the phosphate group of an organic substance under aqueous conditions are described. The labeling compound includes any kind of detectable signal group covalently bound to an imidazole moiety, which can be imidazole or a substituted imidazole. A preferred labeling compound has the formula ##STR1##

Research Organization:
Northeastern Univ., Boston, MA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-90ER60964
Assignee:
Northeastern University (Boston, MA)
Patent Number(s):
5,512,486
Application Number:
08/060,569
OSTI ID:
870396
Resource Relation:
Patent File Date: 1993 May 10
Country of Publication:
United States
Language:
English

References (5)

Solution-phase detection of polynucleotides using interacting fluorescent labels and competitive hybridization journal December 1989
Derivatization of unprotected polynucleotides journal January 1983
Nitrogen-bound and carbon-bound imidazole complexes of ruthenium ammines journal January 1974
Preparation of ethylenediaminephosphoramidates of nucleotides and derivatization with fluorescein isothiocyanate journal July 1990
Modification of Oligo (Poly) Nucleotide Phosphomonoester Groups in Aqueous Solutions journal December 1987

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Related Subjects

single
step
signal
group-imidazole
labeling
organic
phosphate
aqueous
conditions
compounds
methods
covalent
substance
described
compound
detectable
covalently
bound
imidazole
moiety
substituted
preferred
formula
str1
covalently bound
single step
organic phosphate
organic substance
aqueous conditions
/436/999/