Phosphate-specific fluorescence labeling under aqueous conditions

Journal Article · Tue Nov 30 23:00:00 EST 1993 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00071a032· OSTI ID:7236079

Wang, P; Giese, R W ^[1]

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1. Northeast Univ., Boston, MA (United States)

We introduce a technique which achieves phosphate-specific fluorescence labeling under aqueous conditions. The method is convenient since it is carried out in a single step and the residual reagents are removed by cation-exchange trapping. These collective advantages make the methodology worthy of further attention as a means to detect DNA adducts and some other trace phosphate-analytes. 16 refs., 2 figs.

• DOE Contract Number: FG02-90ER60964
• OSTI ID: 7236079
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 65:23; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400100* -- Analytical & Separations Chemistry
664200 -- Spectra of Atoms & Molecules & their Interactions with Photons-- (1992-)
74 ATOMIC AND MOLECULAR PHYSICS
AQUEOUS SOLUTIONS
CAPILLARY FLOW
CATIONS
CHARGED PARTICLES
CHEMICAL ANALYSIS
DISPERSIONS
ELECTROPHORESIS
FLUID FLOW
FLUORESCENCE
ION EXCHANGE
ION EXCHANGE MATERIALS
IONS
LABELLING
LUMINESCENCE
MATERIALS
MIXTURES
ORGANIC ION EXCHANGERS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS COMPOUNDS
SOLUTIONS