New fluorescence methodology for detecting DNA adducts. Final progress report, May 1, 1991--November 30, 1994
A new reagent, {open_quotes}BO-IMI{close_quotes}, has been developed that achieves, for the first time, single step, phosphate specific fluorescence labeling under aqueous conditions. Both 3{prime} and 5{prime} mononucleotides, including representative DNA adducts can be labeled. Included in this technique is a convenient procedure for postlabeling sample cleanup, leading to a practical detection of the products by capillary electrophoresis with laser fluorescence detection (CE-LIF). We consider that this new method will have a significant impact on the measurement of DNA adducts in human samples. This work was largely accomplished in the second half of our project. In the first half, we set up a new way to isolate DNA nucleotides from blood, worked with an initial, less specific technique for labeling DNA adducts, compared ionizing radiation vs oxidative damage to fluorescein labeled deoxyadenylic acid, and set up a capillary electrophoresis laser fluorescence detection system.
- Research Organization:
- Northeastern Univ., Boston, MA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-90ER60964
- OSTI ID:
- 29367
- Report Number(s):
- DOE/ER/60964--T1; ON: DE95006408
- Country of Publication:
- United States
- Language:
- English
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