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Method for rapid base sequencing in DNA and RNA with two base labeling

Patent ·
OSTI ID:869830
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Los Alamos, NM
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Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used to replicate a single DNA or RNA strand to be sequenced. The bases are then sequentially cleaved from the replicated strand, excited with a chosen spectrum of electromagnetic radiation, and the fluorescence from individual, tagged bases detected in the order of cleavage from the strand.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM
DOE Contract Number:
W-7405-ENG-36
Assignee:
Regents of University of California Office of Technology Transfer (Alameda, CA)
Patent Number(s):
US 5405747
OSTI ID:
869830
Country of Publication:
United States
Language:
English

References (4)

A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides journal October 1987
High-Speed DNA Sequencing: An Approach Based Upon Fluorescence Detection of Single Molecules journal October 1989
Single-molecule detection as an approach to rapid DNA sequencing journal January 1992
Fluorescence detection in automated DNA sequence analysis journal June 1986

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