Method for rapid base sequencing in DNA and RNA

Patent · Sun Dec 31 23:00:00 EST 1989

OSTI ID:867551

Jett, James H ^[1]; Keller, Richard A ^[1]; Martin, John C ^[1]; Moyzis, Robert K ^[1]; Ratliff, Robert L ^[1]; Shera, E Brooks ^[1]; Stewart, Carleton C ^[1]

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1. Los Alamos, NM

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM
• DOE Contract Number: W-7405-ENG-36
• Assignee: United States of America (Washington, DC)
• Patent Number(s): US 4962037
• OSTI ID: 867551
• Country of Publication: United States
• Language: English

Related Subjects

/435/436/
accordingly
base
base sequence
base sequencing
bases
characteristic
cleaved
cleaves
detection
dna
dye
embodiment
excited
exonuclease
flow
flow stream
fluorescence
fluorescent
fluorescent dye
forming
fragment
fragments
identifiable
identification
individual
individually
maintains
method
moving
orderly
original
output
output spectrum
particular
particular embodiment
provided
rapid
rapid base
reconstructed
rna
sequence
sequencing
sequentially
single
spectrum
stream
subsequent
subsequent detection
suspended
tagged
train