Method for rapid base sequencing in DNA and RNA

Patent · Wed Oct 07 00:00:00 EDT 1987

OSTI ID:6864735

Jett, J H; Keller, R A; Martin, J C; Moyzis, R K; Ratliff, R L; Shera, E B; Stewart, C C

A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed. 2 figs.

• Research Organization: Lawrence Berkeley Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-36
• Assignee: LBNL; EDB-88-179642
• Patent Number(s): PATENTS-US-A6105375
• OSTI ID: 6864735
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOLOGY
DNA SEQUENCING
DYES
ENZYMES
ESTERASES
EVALUATION
GENES
GENETICS
HYDROLASES
INVENTIONS
LABELLING
MODIFICATIONS
NUCLEASES
NUCLEIC ACIDS
OPERATION
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
RNA
STRUCTURAL CHEMICAL ANALYSIS