Detection method for dissociation of multiple-charged ions

Patent · Mon Dec 31 23:00:00 EST 1990

OSTI ID:868095

Smith, Richard D ^[1]; Udseth, Harold R ^[1]; Rockwood, Alan L ^[1]

---

1. Richland, WA

Dissociations of multiple-charged ions are detected and analyzed by charge-separation tandem mass spectrometry. Analyte molecules are ionized to form multiple-charged parent ions. A particular charge parent ion state is selected in a first-stage mass spectrometer and its mass-to-charge ratio (M/Z) is detected to determine its mass and charge. The selected parent ions are then dissociated, each into a plurality of fragments including a set of daughter ions each having a mass of at least one molecular weight and a charge of at least one. Sets of daughter ions resulting from the dissociation of one parent ion (sibling ions) vary in number but typically include two to four ions, one or more multiply-charged. A second stage mass spectrometer detects mass-to-charge ratio (m/z) of the daughter ions and a temporal or temporo-spatial relationship among them. This relationship is used to correlate the daughter ions to determine which (m/z) ratios belong to a set of sibling ions. Values of mass and charge of each of the sibling ions are determined simultaneously from their respective (m/z) ratios such that the sibling ion charges are integers and sum to the parent ion charge.

• Research Organization: BATTELLE MEMORIAL INSTITUTE
• DOE Contract Number: AC06-76RL01830
• Assignee: Battelle Memorial Institute (Richland, WA)
• Patent Number(s): US 5073713
• OSTI ID: 868095
• Country of Publication: United States
• Language: English

Related Subjects

/250/
analyte
analyzed
belong
charge
charge-separation
charges
correlate
daughter
detected
detection
detection method
detects
determine
determined
dissociated
dissociation
dissociations
first-stage
form
form multiple
fragments
including
integers
ionized
mass
mass spectrometer
mass spectrometry
mass-to-charge
method
molecular
molecular weight
molecules
multiple-charged
multiply-charged
parent
particular
plurality
ratio
ratios
relationship
respective
resulting
selected
set
sets
sibling
simultaneously
spectrometer
spectrometry
stage
tandem
temporal
temporo-spatial
typically
values
vary
weight
z