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Title: Apparatus and method of determining molecular weight of large molecules

Abstract

A mass spectrometer determines the mass of multiply charged high molecular weight molecules. This spectrometer utilizes an ion detector which is capable of simultaneously measuring the charge z and transit time of a single ion as it passes through the detector. From this transit time, the velocity of the single ion may then be derived, thus providing the mass-to-charge ratio m/z for a single ion which has been accelerated through a known potential. Given z and m/z, the mass m of the single ion can then be calculated. Electrospray ions with masses in excess of 1 MDa and charge numbers greater than 425 e{sup {minus}} are readily detected. The on-axis single ion detection configuration enables a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation. 14 figs.

Inventors:
; ; ;
Publication Date:
Research Org.:
University of California
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
672527
Patent Number(s):
US 5,770,857/A/
Application Number:
PAN: 8-749,837
Assignee:
Univ. of California, Oakland, CA (United States) PTO; SCA: 400102; PA: EDB-98:120299; SN: 98002024882
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 23 Jun 1998
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; MASS SPECTROMETERS; MOLECULAR WEIGHT; DESIGN; OPERATION; MASS

Citation Formats

Fuerstenau, S., Benner, W.H., Madden, N.M., and Searles, W. Apparatus and method of determining molecular weight of large molecules. United States: N. p., 1998. Web.
Fuerstenau, S., Benner, W.H., Madden, N.M., & Searles, W. Apparatus and method of determining molecular weight of large molecules. United States.
Fuerstenau, S., Benner, W.H., Madden, N.M., and Searles, W. Tue . "Apparatus and method of determining molecular weight of large molecules". United States.
@article{osti_672527,
title = {Apparatus and method of determining molecular weight of large molecules},
author = {Fuerstenau, S. and Benner, W.H. and Madden, N.M. and Searles, W.},
abstractNote = {A mass spectrometer determines the mass of multiply charged high molecular weight molecules. This spectrometer utilizes an ion detector which is capable of simultaneously measuring the charge z and transit time of a single ion as it passes through the detector. From this transit time, the velocity of the single ion may then be derived, thus providing the mass-to-charge ratio m/z for a single ion which has been accelerated through a known potential. Given z and m/z, the mass m of the single ion can then be calculated. Electrospray ions with masses in excess of 1 MDa and charge numbers greater than 425 e{sup {minus}} are readily detected. The on-axis single ion detection configuration enables a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation. 14 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 23 00:00:00 EDT 1998},
month = {Tue Jun 23 00:00:00 EDT 1998}
}