Direct charge number and molecular weight determination of large individual ions by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Journal Article · Mon Nov 14 23:00:00 EST 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00094a018· OSTI ID:6718859

Chen, R; Wu, Q; Mitchell, D W; Hofstadler, S A; Rockwood, A L; Smith, R D ^[1]

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1. Pacific Northwest Lab., Richland, WA (United States)

The coupling of electrospray ionization (ESI) techniques with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry allows the analysis of individual (i.e., single) multiply charged ions. In this paper, we demonstrate that individual large ions can be directly characterized through their excitation and ejection behavior in the FTICR cell. We also report the direct measurement of the charge carried by an individual poly(ethylene glycol) ion (5 [times] 10[sup 6] nominal molecular weight) and thus obtain the molecular weight of an individual ion ([approximately] 4.1 [times] 10[sup 6]) directly from the m/z measurement. These results confirm that the observed ions are indeed large individual molecular ions produced by ESI, as opposed to small fragments, and that an approximate molecular weight can be directly measured on the basis of charge determination and measured m/z. This capability augments the ability for more precise mass determination based upon the observation of a known reaction process (e.g., proton transfer) for individual ions. 39 refs., 5 figs., 1 tab.

• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6718859
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 66:22; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
400201 -- Chemical & Physicochemical Properties
ALCOHOLS
CHARGE STATES
CHARGED PARTICLES
CHARGED-PARTICLE TRANSPORT
ENERGY-LEVEL TRANSITIONS
EXCITATION
FOURIER TRANSFORM SPECTROMETERS
GLYCOLS
HYDROXY COMPOUNDS
ION CYCLOTRON RESONANCE SPECTROSCOPY
ION SPECTROSCOPY
IONIZATION
IONS
MASS SPECTRA
MASS SPECTROSCOPY
MEASURING INSTRUMENTS
MOLECULAR IONS
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
ORGANIC POLYMERS
POLYETHYLENE GLYCOLS
POLYMERS
PROTON TRANSPORT
RADIATION TRANSPORT
RESONANCE IONIZATION MASS SPECTROSCOPY
SPECTRA
SPECTROMETERS
SPECTROSCOPY