Combined electrophoretic-separation and electrospray method and system
- Richland, WA
- Kennewick, WA
A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary zone electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- DOE Contract Number:
- AC06-76RL01830
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Number(s):
- US 4842701
- OSTI ID:
- 867018
- Country of Publication:
- United States
- Language:
- English
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Combined electrophoresis-electrospray interface and method
Combined electrophoresis-electrospray interface and method
Related Subjects
electrophoretic-separation
electrospray
method
analyzing
molecular
constituents
composition
sample
forming
solution
separating
capillary
zone
electrophoresis
eluent
longitudinally
separated
according
relative
electrophoretic
mobilities
electrospraying
form
charged
spray
temporal
distribution
detecting
collecting
accordance
high-voltage
5-100
kvdc
applied
applying
voltage
2-8
exit
cathode
spaced
front
complete
electrical
circuit
formed
conductor
directly
contacts
zone electrophoresis
temporal distribution
molecular constituents
electrical circuit
capillary zone
capillary exit
relative electrophoretic
separated according
separated constituents
charged spray
composition sample
complete electrical
cathode spaced
analyzing molecular
electrophoretic mobilities
longitudinally separated
constituents longitudinally
directly contacts
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