Combined electrophoresis-electrospray interface and method
A system and method for analyzing molecular constituents of a composition sample include: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5--100 kVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g.,{+-}2--8 kVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit. 21 figs.
- Research Organization:
- Battelle Memorial Institute
- DOE Contract Number:
- AC06-76RL01830
- Assignee:
- Battelle Memorial Inst., Richland, WA (United States)
- Patent Number(s):
- US RE 34,757/E/
- Application Number:
- PAN: 7-803,145
- OSTI ID:
- 27740
- Country of Publication:
- United States
- Language:
- English
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Combined electrophoresis-electrospray interface and method
Combined electrophoresis-electrospray interface and method