skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Combined electrophoresis-electrospray interface and method

Patent ·
OSTI ID:875295
 [1];  [1];  [2]
  1. Richland, WA
  2. Los Alamos, NM
+ Show Author Affiliations

A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA Battelle Memorial Institute
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
US RE34757
OSTI ID:
875295
Country of Publication:
United States
Language:
English

References (7)

Capillary zone electrophoresis journal October 1983
Electrospray mass spectroscopy of macromolecules: Application of an ion-drift spectrometer journal June 1984
Mixed zone analysis in isotachophoresis with selective detection by mass spectrometry applied to the quantitation of hydrogenation products of aromatic quaternary ammonium compounds journal January 1985
Electrospray interface for liquid chromatographs and mass spectrometers journal March 1985
Electrospray mass spectroscopy of macromolecule degradation in the electrospray journal May 1975
Negative ion production with the electrospray ion source journal September 1984
Electrospray ion source. Another variation on the free-jet theme journal September 1984

Similar Records

Related Subjects

combined
electrophoresis-electrospray
interface
method
analyzing
molecular
constituents
composition
sample
forming
solution
separating
capillary
electrophoresis
eluent
longitudinally
separated
according
relative
electrophoretic
mobilities
electrospraying
form
charged
spray
temporal
distribution
detecting
collecting
accordance
high-voltage
5-100
kvdc
applied
applying
voltage
2-8
exit
cathode
spaced
front
complete
electrical
circuit
formed
conductor
directly
contacts
conduction
sheath
electrode
discharged
annular
flow
temporal distribution
molecular constituents
sheath flow
capillary electrophoresis
electrical circuit
capillary exit
relative electrophoretic
separated according
separated constituents
charged spray
combined electrophoresis-electrospray
composition sample
complete electrical
cathode spaced
analyzing molecular
electrophoresis-electrospray interface
electrophoretic mobilities
longitudinally separated
constituents longitudinally
directly contacts
MIXTURES
ATOMIZATION
SAMPLE PREPARATION
ELECTRIC FIELDS
ELECTROLYTIC CELLS
ELECTRODES
/204/250/