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Combined electrophoresis-electrospray interface and method

Patent ·
OSTI ID:875295
 [1];  [1];  [2]
  1. Richland, WA
  2. Los Alamos, NM
+ Show Author Affiliations

A system and method for analyzing molecular constituents of a composition sample includes: forming a solution of the sample, separating the solution by capillary electrophoresis into an eluent of constituents longitudinally separated according to their relative electrophoretic mobilities, electrospraying the eluent to form a charged spray in which the molecular constituents have a temporal distribution; and detecting or collecting the separated constituents in accordance with the temporal distribution in the spray. A first high-voltage (e.g., 5-100 KVDC) is applied to the solution. The spray is charged by applying a second high voltage (e.g., .+-.2-8 KVDC) between the eluent at the capillary exit and a cathode spaced in front of the exit. A complete electrical circuit is formed by a conductor which directly contacts the eluent at the capillary exit, or by conduction through a sheath electrode discharged in an annular sheath flow about the capillary exit.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA Battelle Memorial Institute
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
US RE34757
OSTI ID:
875295
Country of Publication:
United States
Language:
English

References (7)

Mixed zone analysis in isotachophoresis with selective detection by mass spectrometry applied to the quantitation of hydrogenation products of aromatic quaternary ammonium compounds journal January 1985
Electrospray interface for liquid chromatographs and mass spectrometers journal March 1985
Electrospray mass spectroscopy of macromolecule degradation in the electrospray journal May 1975
Electrospray mass spectroscopy of macromolecules: Application of an ion-drift spectrometer journal June 1984
Capillary zone electrophoresis journal October 1983
Electrospray ion source. Another variation on the free-jet theme journal September 1984
Negative ion production with the electrospray ion source journal September 1984

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Related Subjects

/204/250/
2-8
5-100
ATOMIZATION
ELECTRIC FIELDS
ELECTRODES
ELECTROLYTIC CELLS
ELECTROPHORESIS
MIXTURES
SAMPLE PREPARATION
accordance
according
analyzing
analyzing molecular
annular
applied
applying
capillary
capillary electrophoresis
capillary exit
cathode
cathode spaced
charged
charged spray
circuit
collecting
combined
combined electrophoresis-electrospray
complete
complete electrical
composition
composition sample
conduction
conductor
constituents
constituents longitudinally
contacts
detecting
directly
directly contacts
discharged
distribution
electrical
electrical circuit
electrode
electrophoresis
electrophoresis-electrospray
electrophoresis-electrospray interface
electrophoretic
electrophoretic mobilities
electrospraying
eluent
exit
flow
form
formed
forming
front
high-voltage
interface
kvdc
longitudinally
longitudinally separated
method
mobilities
molecular
molecular constituents
relative
relative electrophoretic
sample
separated
separated according
separated constituents
separating
sheath
sheath flow
solution
spaced
spray
temporal
temporal distribution
voltage