pLS010 plasmid vector

Patent · Thu Dec 31 23:00:00 EST 1987

OSTI ID:866521

Lacks, Sanford A ^[1]; Balganesh, Tanjore S ^[2]

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1. Brookhaven, NY
2. Upton, NY

Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.

• Research Organization: ASSOC UNIVERSITIES INC
• DOE Contract Number: AC02-76CH00016
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4729954
• OSTI ID: 866521
• Country of Publication: United States
• Language: English

Related Subjects

/435/
bacteria
bacterial
consisting
consisting essentially
contains
disclosed
dna
dna fragment
enrichment
essentially
exogeneous
fragment
gram-positive
gram-positive bacteria
inserting
isolation
kb
ligated
malm
method
particularly
particularly useful
penicillin
plasmid
plasmid contains
plasmids
pls010
pls101
processes
recombinant
recombinant plasmid
recombinant plasmids
restriction
restriction site
selection
sequences
sites
suitable
systems
transforming
useful
vector