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Title: pLS010 plasmid vector

Abstract

Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.

Inventors:
 [1];  [2]
+ Show Inventor Affiliations
  1. Brookhaven, NY
  2. Upton, NY
Publication Date:
Research Org.:
Associated Universities, Inc., Upton, NY (United States)
OSTI Identifier:
866521
Patent Number(s):
US 4729954
Assignee:
United States of America as represented by United States (Washington, DC)
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
pls010; plasmid; vector; disclosed; recombinant; pls101; consisting; essentially; kb; malm; fragment; ligated; dna; particularly; useful; transforming; gram-positive; bacteria; contains; restriction; sites; suitable; inserting; exogeneous; sequences; method; isolation; penicillin; selection; processes; enrichment; plasmids; bacterial; systems; consisting essentially; particularly useful; dna fragment; restriction site; recombinant plasmids; recombinant plasmid; gram-positive bacteria; plasmid contains; /435/

Citation Formats

Lacks, Sanford A, and Balganesh, Tanjore S. pLS010 plasmid vector. United States: N. p., 1988. Web.
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Lacks, Sanford A, & Balganesh, Tanjore S. pLS010 plasmid vector. United States.
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Lacks, Sanford A, and Balganesh, Tanjore S. 1988. "pLS010 plasmid vector". United States. https://www.osti.gov/servlets/purl/866521.
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@article{osti_866521,
title = {pLS010 plasmid vector},
author = {Lacks, Sanford A and Balganesh, Tanjore S},
abstractNote = {Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb malM gene fragment ligated to a 4.4 Kb T.sub.c r DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems.},
doi = {},
url = {https://www.osti.gov/biblio/866521}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}
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