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U.S. Department of Energy
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pLS101 plasmid vector

Patent ·
OSTI ID:5528773

Disclosed is recombinant plasmid pLS101, consisting essentially of a 2.0 Kb ma1M gene fragment ligated to a 4.4 Kb Tcr DNA fragment, which is particularly useful for transforming Gram-positive bacteria. This plasmid contains at least four restriction sites suitable for inserting exogeneous gene sequences. Also disclosed is a method for plasmid isolation by penicillin selection, as well as processes for enrichment of recombinant plasmids in Gram-positive bacterial systems. 5 figs., 2 tabs.

Research Organization:
Brookhaven National Lab., Upton, NY (USA)
DOE Contract Number:
AC02-76CH00016
Assignee:
Dept. of Energy
Patent Number(s):
None
Application Number:
ON: DE86013777
OSTI ID:
5528773
Country of Publication:
United States
Language:
English

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