Method of saccharifying cellulose

Patent · Tue Sep 10 00:00:00 EDT 1985

OSTI ID:865590

Johnson, Eric A ^[1]; Demain, Arnold L ^[2]; Madia, Ashwin ^[3]

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1. Brookline, MA
2. Wellesley, MA
3. Decatur, IL

A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.

• Research Organization: Midwest Research Institute
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4540664
• Application Number: 06/494,482
• OSTI ID: 865590
• Country of Publication: United States
• Language: English

Related Subjects

/435/
advantageously
agent
alkaline
alkaline earth
amount
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cellulase
cellulose
clostridium
clostridium thermocellum
completely
concentration
containing
controlled
cotton
crystalline
crystalline cellulose
duration
earth
earth salts
efficacious
found
incubation
method
native
parameters
ph
reducing
reducing agent
saccharification
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saccharifying
saccharifying cellulose
salts
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temperature
thermocellum