Method of saccharifying cellulose

Patent · Tue Sep 10 00:00:00 EDT 1985

OSTI ID:6036407

Johnson, E A; Demain, A L; Madia, A

A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.

• Assignee: The United States of America as represented by the United States Department of Energy
• Patent Number(s): US 4540664
• OSTI ID: 6036407
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400301* -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
ALKALINE EARTH METALS
BACTERIA
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
CLOSTRIDIUM
CLOSTRIDIUM THERMOCELLUM
COTTON
DECOMPOSITION
ELEMENTS
HALIDES
HALOGEN COMPOUNDS
HYDROLYSIS
INCUBATION
LYSIS
METALS
MICROORGANISMS
ORGANIC COMPOUNDS
PH VALUE
POLYSACCHARIDES
REDUCING AGENTS
SACCHARIDES
SACCHARIFICATION
SOLVOLYSIS
TEMPERATURE DEPENDENCE
TIME DEPENDENCE