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Title: Method of saccharifying cellulose

Abstract

A method is disclosed of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of thiol reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.

Inventors:
; ;
Publication Date:
OSTI Identifier:
6981250
Application Number:
ON: DE84011079
Assignee:
Dept. of Energy EDB-84-081093
DOE Contract Number:  
AC02-77ET20030
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; CELLULOSE; ENZYMATIC HYDROLYSIS; CALCIUM COMPOUNDS; CELLULASE; CLOSTRIDIUM THERMOCELLUM; COTTON; PH VALUE; THIOLS; ALKALINE EARTH METAL COMPOUNDS; BACTERIA; CARBOHYDRATES; CHEMICAL REACTIONS; CLOSTRIDIUM; DECOMPOSITION; ENZYMES; GLYCOSYL HYDROLASES; HYDROLASES; HYDROLYSIS; LYSIS; MICROORGANISMS; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)

Citation Formats

Johnson, E.A., Demain, A.L., and Madia, A.. Method of saccharifying cellulose. United States: N. p., 1983. Web.
Johnson, E.A., Demain, A.L., & Madia, A.. Method of saccharifying cellulose. United States.
Johnson, E.A., Demain, A.L., and Madia, A.. Fri . "Method of saccharifying cellulose". United States. doi:.
@article{osti_6981250,
title = {Method of saccharifying cellulose},
author = {Johnson, E.A. and Demain, A.L. and Madia, A.},
abstractNote = {A method is disclosed of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of thiol reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri May 13 00:00:00 EDT 1983},
month = {Fri May 13 00:00:00 EDT 1983}
}