Method of saccharifying cellulose

Patent · Fri May 13 00:00:00 EDT 1983

OSTI ID:6981250

Johnson, E A; Demain, A L; Madia, A

A method is disclosed of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of thiol reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.

• DOE Contract Number: AC02-77ET20030
• Assignee: Dept. of Energy
• Patent Number(s): None
• Application Number: ON: DE84011079
• OSTI ID: 6981250
• Country of Publication: United States
• Language: English

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ALKALINE EARTH METAL COMPOUNDS
BACTERIA
CALCIUM COMPOUNDS
CARBOHYDRATES
CELLULASE
CELLULOSE
CHEMICAL REACTIONS
CLOSTRIDIUM
CLOSTRIDIUM THERMOCELLUM
COTTON
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PH VALUE
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
THIOLS