Substrates and method for determining enzymes

Patent · Thu Jan 01 04:00:00 EST 1981

OSTI ID:864026

Smith, Robert E ^[1]; Bissell, Eugene R ^[2]

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1. 574 Escondido Cir., Livermore, CA 94550
2. 101 Via Lucia, Alamo, CA 94507

A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• DOE Contract Number: W-7405-ENG-48
• Assignee: Smith, Robert E. (574 Escondido Cir., Livermore, CA 94550);Bissell, Eugene R. (101 Via Lucia, Alamo, CA 94507)
• Patent Number(s): US 4294923
• OSTI ID: 864026
• Country of Publication: United States
• Language: English

Related Subjects

/435/930/
7-amino-4-trifluoromethylcoumarin
acid
acid derivative
amino
amino acid
biological
biological fluid
chromogenic
chromophore
contacting
containing fluid
derivative
determining
disclosed
effect
enzymatic
enzymatic hydrolysis
enzyme
enzymes
fluid
fluorometrically
free
hydrolysis
hydrolyzate
incubating
method
presence
steps
substrate
substrate-containing
substrates
synthetic