Direct photometric or fluorometric assay of proteinases using substrates containing 7-amino-4-trifluoromethylcoumarin

Journal Article · Tue Jan 01 04:00:00 EST 1980 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(80)90074-2· OSTI ID:6319260

Smith, R E; Bissell, E R; Mitchell, A R; Pearson, K W

N-Acyl derivatives of 7-amino-4-trifluoromethylcoumarin (I) provide new and novel substrates for the assay of proteinases, allowing either direct fluorometric or photometric measurements. The yellow-green fluorescence of the free coumarin I makes it possible to visually observe hydrolysis of the arylamide bond, thus making it a very useful non-mutagenic fluorophore for the identification and quantification of proteinases whether in dynamic assay or in intact cells.

• Research Organization: Univ. of California, Livermore
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6319260
• Journal Information: Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 17; ISSN THBRA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
COUMARIN
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ESTERS
FLUORESCENCE
FLUORINE COMPOUNDS
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROLYSIS
KINETICS
LACTONES
LUMINESCENCE
LYSIS
MUTAGENESIS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PROTEINS
PYRANS
REACTION KINETICS
SOLVOLYSIS
SPECTROPHOTOMETRY
SUBSTRATES