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Title: Substrates and method for determining enzymes

Abstract

A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate. No Drawings

Inventors:
;
Publication Date:
OSTI Identifier:
7254143
Patent Number(s):
US 4294923; A
Application Number:
PPN: US 6-032444
Assignee:
PTO; EDB-94-125890
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 23 Apr 1979
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ENZYMES; MEASURING METHODS; BIOCHEMISTRY; BODY FLUIDS; ENZYMATIC HYDROLYSIS; BIOLOGICAL MATERIALS; CHEMICAL REACTIONS; CHEMISTRY; DECOMPOSITION; HYDROLYSIS; LYSIS; MATERIALS; ORGANIC COMPOUNDS; PROTEINS; SOLVOLYSIS; 550200* - Biochemistry

Citation Formats

Smith, R.E., and Bissell, E.R.. Substrates and method for determining enzymes. United States: N. p., 1981. Web.
Smith, R.E., & Bissell, E.R.. Substrates and method for determining enzymes. United States.
Smith, R.E., and Bissell, E.R.. Tue . "Substrates and method for determining enzymes". United States.
@article{osti_7254143,
title = {Substrates and method for determining enzymes},
author = {Smith, R.E. and Bissell, E.R.},
abstractNote = {A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate. No Drawings},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 13 00:00:00 EDT 1981},
month = {Tue Oct 13 00:00:00 EDT 1981}
}