Substrates and method for determining enzymes
Patent
·
OSTI ID:7254143
A method is disclosed for determining the presence of an enzyme in a biological fluid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4-trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4-trifluoromethylcoumarin chromophore in the hydrolyzate. No Drawings
- DOE Contract Number:
- W-7405-ENG-48
- Assignee:
- PTO; EDB-94-125890
- Patent Number(s):
- A; US 4294923
- Application Number:
- PPN: US 6-032444
- OSTI ID:
- 7254143
- Country of Publication:
- United States
- Language:
- English
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