Aerosol matrix-assisted laser desorption ionization. Effects of analyte concentration and matrix-to-analyte ratio

Journal Article · Sat Jul 01 04:00:00 EDT 1995 · Analytical Chemistry (Washington)

DOI:https://doi.org/10.1021/ac00109a012· OSTI ID:81350

Beeson, M D; Murray, K K; Russell, D H ^[1]

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1. Texas A&M Univ., College Station, TX (United States)

We have recently developed an aerosol-liquid introduction interface for matrix-assisted laser desorption ionization (MALDI) mass spectrometry. In this study, we examine the effect of matrix-to-analyte ratio and analyte concentration on analyte ion yield. These studies were performed using bradykinin, gramicidin S, bovine insulin, and myoglobin as analytes and {alpha}-cyano-4-hydroxycinnamic acid and 4-nitroaniline as matrices. The optimum matrix-to-analyte molar ratio for aerosol MALDI was determined to be 10-100:1, which is lower than that typically used for conventional surface MALDI (100-10 000:1). The ion yield was found to be a nonlinear function of analyte concentration. Possible explanations for these observations are discussed. 43 refs., 3 figs., 1 tab.

• Sponsoring Organization: USDOE
• DOE Contract Number: FG05-85ER13434
• OSTI ID: 81350
• Journal Information: Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 13 Vol. 67; ISSN ANCHAM; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
42 ENGINEERING
55 BIOLOGY AND MEDICINE
BASIC STUDIES
ABUNDANCE
AEROSOLS
BRADYKININ
INSULIN
IONIZATION
LASERS
LIQUIDS
MASS SPECTRA
MASS SPECTROSCOPY
MYOGLOBIN
NITRO COMPOUNDS
ORGANIC COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
USES