Liquid sample introduction for matrix-assisted desorption

Journal Article · Wed Sep 15 04:00:00 EDT 1993 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00066a023· OSTI ID:7015472

Murray, K K; Russell, D H ^[1]

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1. Texas A M Univ., College Station, TX (United States)

One of the most important tools in analytical chemistry is liquid chromatography with mass spectrometric detection (LC/MS). The development of interfaces between liquid separation and mass spectrometry is a difficult problem because the large volume flow of liquid solvent in the LC effluent is incompatible with the high vacuum requirements of a mass spectrometer. In this paper initial results are presented for a new liquid introduction method for a mass spectrometer based on the application of matrix assisted laser desorption ionization (MALDI) to aerosols. Details of the method will be presented in future publications. Aerosols are formed using a continuous pneumatic aerosol generator from an acidified methanol solution containing the matrix and analyte. The solvent is removed by passing the aerosol through a heated tube to form a collimated beam of dried particles. Ions are formed by irradiating the aerosol with pulsed UV radiation in the source region of a linear TOF MS. The advantages of the aerosol MALDI TOF method are (1) liquid flow capacity of 0.5 mL/min, (2) rapid acquisition of the entire mass spectrum, and (3) ionization of large peptides and proteins. In this paper, the aerosol MALDI mass spectra of gramicidin S and lysozyme (chicken egg white) are presented. Flow injection mass spectra of gramicidin S are presented to demonstrate the feasibility of coupling aerosol MALDI with HPLC. 22 refs., 6 figs.

• DOE Contract Number: FG05-85ER13434
• OSTI ID: 7015472
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 65:18; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
400201* -- Chemical & Physicochemical Properties
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AEROSOLS
ALCOHOLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CHROMATOGRAPHY
COLLOIDS
DESORPTION
DISPERSIONS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROXY COMPOUNDS
IONIZATION
LASERS
LIQUID COLUMN CHROMATOGRAPHY
LYSOZYME
MASS SPECTROSCOPY
METHANOL
MIXTURES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PROTEINS
RADIATIONS
SEPARATION PROCESSES
SOLS
SOLUTIONS
SORPTION
SPECTROSCOPY
ULTRAVIOLET RADIATION