Aerosol matrix-assisted laser desorption ionization for liquid chromatography/time-of-flight mass spectrometry

Journal Article · Sun May 15 00:00:00 EDT 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00082a003· OSTI ID:7070297

Murray, K K; Lewis, T M; Beeson, M D; Russell, D H ^[1]

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1. Texas A M Univ., College Station, TX (United States)

We report the application of aerosol matrix-assisted laser desorption ionization (MALDI) to liquid chromatography/mass spectrometry (LC/MS). The aerosol MALDI experiment uses aerosol liquid introduction in conjunction with pulsed UV laser ionization to form ions from large biomolecules in solution. Mass analysis is achieved in a time-of-flight mass spectrometer. In the LC/MALDI-MS experiment, the matrix solution is combined with the column effluent in a mixing tee, LC/MALDI-MS is demonstrated for the separation of bradykinin, gramicidin S, and myoglobin. 32 refs., 8 figs., 1 tab.

• DOE Contract Number: FG05-85ER13434
• OSTI ID: 7070297
• Journal Information: Analytical Chemistry (Washington); (United States), Vol. 66:10; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
ANTIBIOTICS
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
BRADYKININ
MYOGLOBIN
AEROSOLS
COMPUTERS
DESORPTION
IONIZATION
LASERS
TIME-OF-FLIGHT METHOD
ANTI-INFECTIVE AGENTS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
COLLOIDS
DISPERSIONS
DRUGS
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KININS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PIGMENTS
POLYPEPTIDES
PORPHYRINS
PROTEINS
SEPARATION PROCESSES
SOLS
SORPTION
SPECTROSCOPY
400102* - Chemical & Spectral Procedures
400105 - Separation Procedures
550200 - Biochemistry