Effects of radiophosphorus decay in bacteriophage T4D. II. The mechanism of marker rescue. [/sup 32/P]

Journal Article · Sat Nov 01 04:00:00 EST 1975 · Virology; (United States)

DOI:https://doi.org/10.1016/0042-6822(75)90143-9· OSTI ID:7365161

Levy, J N

When bacteriophage T4D is inactivated by decay of incorporated /sup 32/P, about 40 percent of the phage lethal hits (PLH) completely destroy the ability of the damaged phage to contribute genes to the progeny of a mixed infection with a genetically distinct and viable helper phage. The other 60 percent of the PLH inactivate some but not all of the phage genome. The inactivated portions, or knockouts, vary from less than 5 percent to more than 95 percent of the genome in size. Not all portions of the genome are equally sensitive to /sup 32/P decay. The topography of marker rescue from /sup 32/P-damaged phage is similar to that previously observed for ultraviolet-irradiated T4. This topography cannot result from the initial distribution of lethal damages (double-strand breaks), since these are produced at random positions by /sup 32/P decay. It is shown that the topography of rescue also does not result from preferential injection of the regions of high rescue, nor is it due to high recombination frequencies in these regions. Other models that can explain the rescue topography are presented.

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 7365161
• Journal Information: Virology; (United States), Journal Name: Virology; (United States) Vol. 68:1; ISSN VIRLA
• Country of Publication: United States
• Language: English

Related Subjects

560164* -- Radionuclide Effects-Internal Source-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DAYS LIVING RADIOISOTOPES
DECAY
INACTIVATION
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIATION EFFECTS
RADIOISOTOPES
VIRUSES