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Tritium incorporation at specific positions in benzo(a)pyrene

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)

The carcinogen, benzo(a)pyrene, was tritiated by two methods: exchange reaction with T/sub 2/SO/sub 4/ at the 1, 3 and 6 positions and quenching of a Grignard reagent prepared from 6-iodo-benzo(a)pyrene with T/sub 2/O which gave almost exclusively 6-tritiated material. The exchange reaction incorporated 118 mCi/mmole of which 60 percent was determined to be in the 6 position and 40 percent in the 1 and 3 positions. The Grignard reaction produced a specific activity of 56 mCi/mmole of which 97.5 percent was incorporated in the 6 position.

Research Organization:
Univ. of California, Berkeley
OSTI ID:
7362525
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 63:3; ISSN BBRCA
Country of Publication:
United States
Language:
English