Induction of anti-hapten antibody response by hapten--isologous carrier conjugate. II. Specificity of hapten-reactive helper T cells. [X radiation]

Journal Article · Tue Jul 01 04:00:00 EDT 1975 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(75)90068-4· OSTI ID:7362350

Yamashita, U; Takami, T; Kitagawa, M

Anti-hapten antibody production was elicited by the immunization of haptenisologous carrier conjugate (PAB-MGG) in mice. Spleen and lymph node cells taken from these primed mice could demonstrate their helper activity for anti-DNP antibody production when transferred intravenously into 600 R x-irradiated recipient mice along with DNP-primed B cells and the double hapten conjugated carrier, DNP-MGG-PAB. Isologous carrier (MGG)-primed cells could not demonstrate this helper activity. Accordingly, helper cells reactive for a haptenic group are considered to develop by the immunization of hapten-isologous carrier conjugate. Hapten-reactive helper activity was also induced by the immunization of other hapten-isologous carrier conjugates, e.g., MAB-MGG, PABS-MGG or PAB-MSA. These hapten-reactive helper cells were T lymphocytes, as the helper activity of PAB-MGG-primed cells was completely abolished by in vivo ATS-treatment. When the specificity restrictions of T and B cells to the same haptenic group were compared by responsiveness measured after the antigenic stimulation (B cell function by anti-hapten antibody production and T cell function by helper activity), differences were noted, as PAB-MGG-primed T cells could respond not only to DNP-MGG-PAB but also fairly well to DNP-MGG-MAB to demonstrate their helper activity, but PAB-MGG-primed B cells responded to only PAB-MGG. Thus, hapten specificity appears to be much more restricted for B cells than T cells. The difference of this responsivity between B cells and helper T cells was thought to derive from the specificity difference of B cell and helper T cell receptors rather than from any sensitivity differences of the experimental procedure. The differences in the specificity restrictions of receptors of B and helper T cells were discussed in the light of hapten-specificity.

• Research Organization: Osaka Univ.
• OSTI ID: 7362350
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 18:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IMMUNE REACTIONS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MICE
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
THYMOCYTES
VERTEBRATES
X RADIATION