Role of lipid in the induction of hapten-specific delayed hypersensitivity and contact sensitivity

Journal Article · Mon Apr 01 04:00:00 EDT 1974 · J. Immunol.; (United States)

OSTI ID:5340860

Dailey, M O; Hunter, R L

Guinea pigs immunized with bovine serum albumin conjugated with dinitrophenol (BSA-DNP), in Freund's complete adjuvant, produce antibody to the DNP and delayed-type hypersensitivity (DTH) to the BSA. We report that immunization with BSA-DNP doubly conjugated with medium chain fatty acids (L-BSA-DNP) stimulates the production of DTH specific for the hapten DNP with minimal antibody to DNP and no detectable immune response to the carrier BSA. The production of hapten-specific DTH reactions was assessed by two methods. First, animals immunized with L-BSA-DNP produced typical contact sensitivity reactions to epidermal challenge with dinitrofluorobenzene. Second, they produced grossly and microscopically typical delayed hypersensitivity reactions to the intradermal injection of DNP conjugated either to BSA or to a non-cross-reacting carrier, hen egg albumin. In further experiments, DNP-specific DTH was produced by animals immunized with BSA-DNP to which lipid groups had been attached by electrostatic rather than covalent bonds. Evidence is discussed which indicates that the production of DTH to haptens and proteins is largely a function of the lipophilic nature of the entire immunogen and is not dependent upon properties of individual antigenic determinants. Autoradiographic studies with /sup 125/I-labeled material demonstrated that the lipid-rich antigens are localized in the paracortical area of lymph nodes, in close proximity to the thymus-derived cells which are stimulated in the induction of DTH.

• Research Organization: Univ. of Chicago
• OSTI ID: 5340860
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 112:4; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODY FORMATION
ANTIGEN-ANTIBODY REACTIONS
AROMATICS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FLUORINATED AROMATIC HYDROCARBONS
GLOBULINS
GLOBULINS-ALPHA
GUINEA PIGS
HALOGENATED AROMATIC HYDROCARBONS
HAPTOGLOBINS
IMMUNITY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LIPIDS
MAMMALS
METABOLISM
MUCOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
SENSITIVITY
SYNTHESIS
VERTEBRATES