Antibody formation in mouse bone marrow. IV. The influence of splenectomy on the bone marrow plaque-forming cell response to sheep red blood cells. [X Radiation]

Journal Article · Wed Oct 01 04:00:00 EDT 1975 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(75)90201-4· OSTI ID:7362348

Benner, R; van Oudenaren, A

Mouse bone marrow is barely capable of plaque-forming cell (PFC) activity during the primary response to sheep red blood cells (SRBC). However, during the secondary response, it becomes the major center of activity containing IgM-, IgG- and IgA-PFC. In the present paper the influence of splenectomy was studied on primary and secondary PFC activity in the bone marrow. Differences in primary and secondary bone marrow PFC responses are probably related to the presence of B and T memory cells in situ. Therefore the effect of splenectomy on the appearance of B and T memory cells in the bone marrow was also investigated. iv.plenectomy before intravenous (iv) immunization with 4 x 10/sup 8/ SRBC prevented any primary PFC activity in the bone marrow. The influence of splenectomy before priming on secondary PFC activity in the bone marrow depended on the priming dose of SRBC. Splenectomy before priming with 10/sup 7/ SRBC iv completely prevented IgM-, IgG-, and IgA-PFC activity in the bone marrow upon subsequent boosting with 4 x 10/sup 8/ SRBC iv. By means of cell transfer experiments it was shown that after splenectomy no B or T memory cells appeared in the bone marrow after priming with 10/sup 7/ SRBC iv. Cell transfer experiments showed that splenectomy before priming with 10/sup 7/ SRBC iv not only interfered with the appearance of B and T memory cells in the bone marrow, but also with the appearance of B memory cells in peripheral lymph nodes, mesenteric lymph node, Peyer's patches, thymus, and blood. Immunization of spenectomized mice with 4 x 10/sup 8/ SRBC iv induced the appearance of B memory cells in peripheral lymph nodes, mesenteric lymph node, Peyer's patches, thymus, and blood.

• Research Organization: Erasmus Univ., Rotterdam
• OSTI ID: 7362348
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 19:2; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IONIZING RADIATIONS
MAMMALS
MEDICINE
MICE
ORGANS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SPLENECTOMY
SURGERY
TISSUES
VERTEBRATES
X RADIATION