Antibody formation in mouse bone marrow. II. Evidence for a memory-dependent phenomenon. [X radiation]

Journal Article · Mon Jul 01 04:00:00 EDT 1974 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(74)90230-5· OSTI ID:7349010

Benner, R; Meima, F; van der Meulen, G M

Mouse bone marrow is barely capable of plaque-forming cell (PFC) activity in a primary response to sheep red blood cells (SRBC), while PFC activity in the secondary response to SRBC can be clearly demonstrated. This phenomenon was studied by means of cell transfer experiments. T cells, which are involved in an anti-SRBC PFC response, were shown to be very scarce in normal mouse bone marrow. This is considered to be the cause of the low PFC activity in the marrow during the primary response to SRBC. In normal mouse bone marrow precursors of IgM-PFC but not of IgG- and IgA-PFC could be found. Priming with SRBC induced the appearance of IgM-, IgG-, IgA- and T-memory cells in the marrow. These B- and T-memory cells were shown to be specific for the antigen which induced their appearance. It is thought that after a second injection of SRBC the IgM-, IgG- and IgA-memory cells can differentiate with the help of the T-memory cells within the bone marrow into IgM-, IgG- and IgA-PFC respectively. The sequence of appearance of the B-memory cells in the bone marrow was shown to be IgM--IgG--IgA. Six months after the intravenous injection of SRBC, the presence of B-memory cells could be demonstrated not only in spleen and bone marrow, but also in peripheral lymph nodes, mesenteric lymph node, Peyer's patches, thymus and blood. The increase in amount of B-memory cells was most prominent in the spleen.

• Research Organization: Erasmus Univ., Rotterdam
• OSTI ID: 7349010
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 13:1; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODY FORMATION
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BODY
BODY FLUIDS
BONE MARROW
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IONIZING RADIATIONS
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
MICE
ORGANS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SPLEEN
THYMOCYTES
THYMUS
TISSUES
VERTEBRATES
X RADIATION