Cytological detection of cervical carcinoma with new cytochemical markers and flow microanalysis. [Mithramycin, chromomycin]
Conference
·
OSTI ID:7350779
Human cervical cell samples have been stained with mithramycin or chromomycin A3 in an effort to analyze such preparations for premalignant abnormal cells by flow cytometry. Fluorescence from mithramycin or chromomycin A3-stained cells is similar to the fluorescence from DNA in solution when it is complexed with these same antibiotics. Mithramycin or chromomycin A3-stained cells exhibit nuclear specific fluorescence which, for exponentially grown tissue culture cells, reflects the cellular DNA content. All these facts indicate that DNA is the sole intracellular binding site for these antibiotics. Flow cytofluorometry on mithramycin or chromomycin A3-stained cervical cells using a single parameter, fluorescence intensity per cell, appears to be a poor diagnostic procedure. However, simultaneous analysis of cellular fluorescence and small angle light scatter permits a relatively detailed description of each cell sample and appears to be useful for automated sample diagnosis. Qualitative diagnostic analysis based on comparisons of two parameter histograms agrees moderately well with cytomorphological diagnosis on the same cell samples. A technique for quantitation in comparing two parameter histograms is presented and promises to be useful for further progress in flow analysis of human cervical cell samples.
- Research Organization:
- California Univ., Livermore (USA). Lawrence Livermore Lab.; California Univ., San Francisco (USA)
- OSTI ID:
- 7350779
- Report Number(s):
- UCRL-77469; CONF-760445-1
- Country of Publication:
- United States
- Language:
- English
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