Relationships between thrombopoiesis and erythropoiesis: with studies of the effects of preparations of thrombopoietin and erythropoietin. [/sup 59/Fe and /sup 75/Se tracer techniques]

Journal Article · Fri Oct 01 04:00:00 EDT 1976 · Blood; (United States)

OSTI ID:7349191

Evatt, B L; Spivak, J L; Levin, J

The effects of administration of partially purified human urinary erythropoietin and rabbit thrombopoietin, and of endogenously produced erythropoietin and thrombopoietin on both red cell and platelet production were examined in mice. Partially purified thrombopoietin was prepared from rabbit plasma by sequential fractionation with ammonium sulfate precipitation, and DEAE and Sephadex G-100 chromatography. Preparations of thrombopoietin and partially purified human urinary erythropoietin (NIH No. H-11-TaLSL) were administered subcutaneously to normal mice, and the rate of incorporation of selenomethionine-/sup 75/Se into platelets was measured as an index of thrombopoietic activity of the infused material. Erythropoietin and thrombopoietin were assayed for erythropoietic activity by measuring the rate of appearance of /sup 59/Fe in the red cells of posthypoxic polycythemic mice. Preparations containing thrombopoietin had barely measurably erythropoietic acitivity, and 7 units of partially purified erythropoietin had little thrombopoietic activity. When endogenous levels of erythropoietin were increased by hypoxia, platelet production was not enhanced. Similarly, increased levels of thrombopoietin, induced in response to thrombocytopenia produced by platelet antiserum, did not alter red cell production. These data suggest that physiologically increased levels of thrombopoietin do not stimulate erythropoiesis, and that physiologically increased levels of erythropoietin do not stimulate thrombopoiesis. However, currently available, partially purified preparations of erythropoietin and thrombopoietin may be capable of stimulating both platelet and red cell production if used in sufficient quantities.

• Research Organization: Johns Hopkins Univ., Baltimore
• OSTI ID: 7349191
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 48:4; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD CELLS
BLOOD FORMATION
BLOOD PLASMA
BLOOD PLATELETS
BODY FLUIDS
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROPOIESIS
ERYTHROPOIETIN
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MAN
MICE
NUCLEI
PRIMATES
RABBITS
RADIOISOTOPES
RODENTS
SELENIUM 75
SELENIUM ISOTOPES
SEPARATION PROCESSES
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES