Effects of hemorrhage, hypoxia, and a preparation of erythropoietin on thrombopoiesis

Journal Article · Wed Dec 01 04:00:00 EST 1976 · J. Lab. Clin. Med.; (United States)

OSTI ID:5237416

Shreiner, D P; Levin, J

The effects of hemorrhage, hypoxia, or a preparation of erythropoietin on platelet production were investigated by measuring incorporation of selenomethionine-/sup 75/Se (/sup 75/SeM) into the platelets of rabbits or mice. Rabbits that were bled daily for 5 days had a significant increase in the platelet count, 48 hours after cessation of hemorrhage, that coincided with a threefold increase in isotope incorporation into platelets. Mice that were bled daily for 3 days also had significantly higher platelet counts and a 38 percent increase in incorporation of isotope into platelets, 3 days after the last hemorrhage. Normal rabbits, injected with plasma from repeatedly bled, anemic, and moderately thrombocytopenic rabbits, had a 58 percent greater maximum incorporation of /sup 75/SeM than did control animals. Mice exposed to hypoxia for 6 days had a mean platelet count 23 percent lower than normal controls, but no change in incorporation of /sup 75/SeM into platelets. Plasma from hypoxic mice did not stimulate platelet production when injected into normal mice. A preparation of human urinary erythropoietin (15 to 30 U. per mouse or 30 to 120 U. per rabbit) significantly increased incorporation of isotope into the platelets of normal mice and rabbits. The results demonstrated that hemorrhage, but not hypoxia, is associated with increased thrombopoietic activity in plasma. However, large doses of preparations of human erythropoietin contained detectable thrombopoietic activity.

• OSTI ID: 5237416
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 88:6; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANOXIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BLOOD PLATELETS
BODY
BODY FLUIDS
BONE MARROW
DISEASES
ERYTHROPOIETIN
HEMATOLOGY
HEMATOPOIETIC SYSTEM
HEMORRHAGE
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEDICINE
ORGANS
PATHOLOGICAL CHANGES
PHYSIOLOGY
RABBITS
REACTION KINETICS
RESPONSE MODIFYING FACTORS
SYMPTOMS
SYNTHESIS
THROMBOPOIESIS
TISSUES
TRACER TECHNIQUES
VERTEBRATES