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Detection of thrombopoietic activity in plasma by stimulation of suppressed thrombopoiesis

Journal Article · · Journal of Clinical Investigation
DOI:https://doi.org/10.1172/jci106388· OSTI ID:4106619
 [1];  [1]
  1. Johns Hopkins Univ., Baltimore, MD (United States)
Rabbits in which thrombocytosis had been produced by five daily transfusions of platelet concentrates had suppressed endogenous thrombopoiesis, as reflected by decreased incorporation of selenomethionine-75Se (75SeM) into the circulating platelet mass. Rabbits in which endogenous thrombopoiesis had been suppressed by transfusion-induced thrombocytosis were used to detect thrombopoietic activity in rabbit plasma. Thrombopoietic activity was demonstrated in the plasma of both normal and thrombocytopenic donor rabbits. A dose response relationship was observed between the incorporation of 75SeM into platelets and the dose of plasma administered. Infusion of 20-150 ml of plasma from thrombocytopenic donors increased the incorporation of 75SeM into platelets from 52 to 107% above control values. Further, a dose response effect also was seen after infusion of normal plasma, but normal plasma produced less effect than comparable doses of plasma from thrombocytopenic donors.
Research Organization:
Johns Hopkins Univ., Baltimore, MD (United States)
Sponsoring Organization:
US Atomic Energy Commission (AEC)
NSA Number:
NSA-24-050283
OSTI ID:
4106619
Report Number(s):
NYO--1208-105
Journal Information:
Journal of Clinical Investigation, Journal Name: Journal of Clinical Investigation Journal Issue: 9 Vol. 49; ISSN 0021-9738
Publisher:
American Society for Clinical InvestigationCopyright Statement
Country of Publication:
United States
Language:
English