Partial purification of thrombopoietin from the plasma of thrombocytopenic rabbits

Journal Article · Wed Aug 01 04:00:00 EDT 1979 · Blood; (United States)

OSTI ID:5797011

Evatt, B L; Levin, J; Algazy, K M

Partially purified thrombopoiesis-stimulating activity was prepared from the plasma of thrombocytopenic rabbits using ammonium sulfate precipitation and DEAE cellulose, Sephadex, and carboxymethyl cellulose chromatography. The protein fraction precipitated by an ammonium sulfate saturation of 60 to 80%, previously shown to contain thrombopoiesis-stimulating activity, was used as starting material. Column chromatography was carried out at room temperature at pH 5.6. Under these conditions, thrombopoiesis-stimulating activity (thromboprotein) was retained by DEAE cellulose (0/03 M citrate-phosphate buffer) and carboxymethyl cellulose (0/003 M citrate-phosphate buffer), and eluted with 0.4 M NaCl. Thrombopoietin was approximately 1000-fold purified following sequential chromatography with DEAE and carboxymethyl cellulose. Although the three fractions described reproducibly stimulated thrombopoiesis, as measured by increased levels of selenome-thionine-/sup 75/Se (/sup 75/SeM) in the circulating platelets, platelet counts did not increase.

• Research Organization: Johns Hopkins Univ. School of Medicine and Hospital, Baltimore, MD
• OSTI ID: 5797011
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 54:2; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD FORMATION
BLOOD PLASMA
BODY FLUIDS
CHROMATOGRAPHY
DATA
DATA FORMS
EXPERIMENTAL DATA
INFORMATION
ISOLATED VALUES
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
PURIFICATION
RABBITS
SELENIUM COMPOUNDS
SEPARATION PROCESSES
THROMBOPOIESIS
TRACER TECHNIQUES
VERTEBRATES