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Incorporation of adenosine into ATP: formation of compartmentalized ATP

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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The incorporation of (/sup 3/H)adenosine, (/sup 3/H)adenine, and (/sup 3/H)hypoxanthine into adenine nucleotides of nude (athymic) mouse liver and human hepatoma grown subcutaneously in nude mice was studied. /sup 3/H and /sup 32/P radioactive labeling in vivo of acid-soluble nucleotides followed by chromatographic procedures indicated that, in contrast to (/sup 3/H)adenine and (/sup 3/H)hypoxanthine, (/sup 3/H)adenosine is preferentially incorporated into ATP in comparison with its incorporation into AMP and ADP. This phenomenon, as well as complementing the recently reported 3-fold increase in total cellular ATP upon treatment with 0.5 to 1.0 mM concentrations of adenosine, indicates the formation from adenosine of compartmentalized ATP that is not produced from either adenine or hypoxanthine. The observed effect is of larger magnitude in the growth-arrested normal liver than in the actively growing tumor.

Research Organization:
John Collins Warren Labs., Boston
OSTI ID:
7346971
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 73:9; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ANIMAL CELLS
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
GLANDS
IN VIVO
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIVER
MAMMALS
MICE
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RIBOSIDES
RODENTS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
UPTAKE
VERTEBRATES