Adenine and guanine nucleotide metabolism during platelet storage at 22 degree C

Journal Article · Wed Oct 31 23:00:00 EST 1990 · Blood (Journal of Hematology); (USA)

OSTI ID:6071832

Edenbrandt, C M; Murphy, S ^[1]

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1. Thomas Jefferson Univ., Philadelphia, PA (USA)

Adenine and guanine nucleotide metabolism of platelet concentrates (PCs) was studied during storage for transfusion at 22 +/- 2 degrees C over a 7-day period using high-pressure liquid chromatography. There was a steady decrease in platelet adenosine triphosphate (ATP) and adenosine diphosphate (ADP), which was balanced quantitatively by an increase in plasma hypoxanthine. As expected, ammonia accumulated along with hypoxanthine but at a far greater rate. A fall in platelet guanosine triphosphate (GTP) and guanosine diphosphate (GDP) paralleled the fall in ATP + ADP. When adenine was present in the primary anticoagulant, it was carried over into the PC and metabolized. ATP, GTP, total adenine nucleotides, and total guanine nucleotides declined more slowly in the presence of adenine than in its absence. With adenine, the increase in hypoxanthine concentration was more rapid and quantitatively balanced the decrease in adenine and platelet ATP + ADP. Plasma xanthine rose during storage but at a rate that exceeded the decline in GTP + GDP. When platelet ATP + ADP was labeled with 14C-adenine at the initiation of storage, half of the radioactivity was transferred to hypoxanthine (45%) and GTP + GDP + xanthine (5%) by the time storage was completed. The isotopic data were consistent with the presence of a radioactive (metabolic) and a nonradioactive (storage) pool of ATP + ADP at the initiation of storage with each pool contributing approximately equally to the decline in ATP + ADP during storage. The results suggested a continuing synthesis of GTP + GDP from ATP + ADP, explaining the slower rate of fall of GTP + GDP relative to the rate of rise of plasma xanthine. Throughout storage, platelets were able to incorporate 14C-hypoxanthine into both adenine and guanine nucleotides but at a rate that was only one fourth the rate of hypoxanthine accumulation.

• OSTI ID: 6071832
• Journal Information: Blood (Journal of Hematology); (USA), Journal Name: Blood (Journal of Hematology); (USA) Vol. 76:9; ISSN BLOOA; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENINES
ADP
AMINES
ANTIMETABOLITES
AROMATICS
ATP
AZAARENES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
DRUGS
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
HYPOXANTHINE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
METABOLISM
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
SEPARATION PROCESSES
STORAGE
THERAPY
TRACER TECHNIQUES
TRANSFUSIONS