Effect of radiation in vitro on erythropoietin action

Journal Article · Tue Jun 01 04:00:00 EDT 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574458· OSTI ID:7343073

Barcos, M; Goldwasser, E

The effect of ionizing radiation on the response to erythropoietin of cultured rat marrow cells, at constant erythropoietin dose and constant cell density, differed according to the end point analyzed. Erythropoietin-induced RNA synthesis (an early response) and induced glucosamine incorporation into stroma (a late response) were relatively radioresistant. Induced hemoglobin synthesis was characterized by marked radiosensitivity. Inactivation parameters were anti n = 1.04 and D/sub 0/ = 63 R (59 rad) +- 15 R (95 percent confidence interval). The radiosensitivity was not the result of inactivation of secondary enzymic processes. The inactivation curves for stimulated hemoglobin synthesis at different doses of erythropoietin near the physiological range were found to be linear, with anti n values in the range of 0.92 to 1.14. The corresponding log-log erythropoietin dose--response plots at different radiation doses were linear and parallel, a result inconsistent with an all-or-none action of radiation on erythropoietin-sensitive cells. At high cell density, the effect of 60 R on induced hemoglobin disappeared, whereas the effect on induced iron uptake persisted.

• Research Organization: Univ. of Chicago
• OSTI ID: 7343073
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 66:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BONE MARROW CELLS
CARBOXYLIC ACIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ELEMENTS
ERYTHROPOIETIN
GAMMA RADIATION
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IN VITRO
IONIZING RADIATIONS
IRON
IRRADIATION
MAMMALS
METALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RATS
RODENTS
SOMATIC CELLS
SYNTHESIS
TRANSITION ELEMENTS
UPTAKE
VERTEBRATES
X RADIATION