Identification by irradiation, in vitro, of two components of erythroprotein action

Journal Article · Wed Feb 01 04:00:00 EST 1978 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574822· OSTI ID:7276942

Barcos, M

The effect of ionizing radiation on the response of normal cultured rat marrow cells to erythropoietin yielded two-component inactivation curves for induced iron uptake and hemoglobin synthesis. The radioresistant component of the induced hemoglobin response (1) was detected earlier, at 6 to 20 hr after irradiation, (2) had a DO/sub 0/ > or = to 900 R, (3) gave a nonlinear erythropoietin dose--response plot at 600 R, (4) disappeared when marrow from erythremic rats was used, and (5) showed maximal inactivation by 500 R when irradiation preceded hormone addition by 1.5 to 2.5 hr. The radiosensitive component (1) was observed without any contribution from the radioresistant component when the time of assay of normal marrow was postponed from 6 to 20 hr to 20 to 44 hr of culture, (2) had a D/sub 0/ = 63 R, (3) gave linear erythropoietin dose--response curves at 15 to 60 R, and (4) showed enhanced inhibition by 60 R if irradiation either preceded or followed hormone addition by 3 hr or more.

• Research Organization: Univ. of Chicago
• OSTI ID: 7276942
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 73:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BONE MARROW CELLS
CARBOXYLIC ACIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ELEMENTS
ERYTHROPOIETIN
GAMMA RADIATION
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
IRON
IRRADIATION
MAMMALS
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RATS
RODENTS
SOMATIC CELLS
SYNTHESIS
TRANSITION ELEMENTS
UPTAKE
VERTEBRATES
X RADIATION