Inactivation of biologically active dna by gamma ray induced superoxide radicals and their dismutation products singlet molecular oxygen and hydrogen peroxide

Technical Report · Tue Dec 31 23:00:00 EST 1974

OSTI ID:7342730

Vanhemmen, J J; Meuling, W J.A.

The reactivity of gamma ray induced superoxide radicals and dismutation products (singlet molecular oxygen and hydrogen peroxide) with DNA were studied. Superoxide dismutase, which removes superoxide radicals and inhibits the formation of singlet oxygen, protects biologically active DNA (OX174 RF) against inactivation by ionizing radiation. Catalase, which removes hydrogen peroxide, also protects the DNA. Attempts with various chemical sources of singlet oxygen to determine whether this species inactivates DNA did not yield an unequivocal answer. It was concluded that a combination of the protonated form of the superoxide radical and hydrogen peroxide inactivates DNA. (Author) (GRA)

• Research Organization: Rijksverdedigingsorganisatie TNO, Rijswijk (Netherlands). Medisch Biologisch Lab. TNO
• OSTI ID: 7342730
• Report Number(s): N-75-33664; MBL-1975-10; TDCK-66036
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CATALASE
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
GAMMA RADIATION
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
IONIZING RADIATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDES
RADIATION EFFECTS
RADIATIONS
RADICALS
RADIOSENSITIVITY EFFECTS